Plasmid_Backbone

Part:BBa_K1438031:Design

Designed by: Johann Bauerfeind   Group: iGEM14_Berlin   (2014-10-10)


pQE_80L


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 88
    Illegal XbaI site found at 1127
    Illegal XbaI site found at 2417
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 88
    Illegal NheI site found at 271
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 88
    Illegal BamHI site found at 145
    Illegal XhoI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 88
    Illegal XbaI site found at 1127
    Illegal XbaI site found at 2417
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 88
    Illegal XbaI site found at 1127
    Illegal XbaI site found at 2417
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 3790
    Illegal SapI site found at 2707


Design Notes

pQE_80L Expression Plasmi


Source

pQE_80L Expression Plasmi

References